##FastQC	0.9.1
>>Basic Statistics	pass
#Measure	Value	
Filename	1000gsample.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5000	
Sequence length	54	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.9946	31.0	27.0	33.0	22.0	34.0
2	28.486	30.0	26.0	32.0	21.0	33.0
3	28.5272	30.0	26.0	32.0	21.0	34.0
4	28.6252	30.0	26.0	32.0	22.0	33.0
5	28.4804	30.0	26.0	32.0	21.0	33.0
6	28.5128	30.0	26.0	32.0	22.0	33.0
7	28.4102	30.0	26.0	32.0	21.0	33.0
8	28.4674	30.0	26.0	32.0	22.0	33.0
9	28.182	30.0	26.0	32.0	21.0	33.0
10	28.0908	30.0	26.0	32.0	21.0	33.0
11	28.1876	30.0	26.0	32.0	21.0	33.0
12	28.1742	30.0	26.0	32.0	21.0	33.0
13	27.923	30.0	25.0	32.0	20.0	33.0
14	27.8984	29.0	25.0	32.0	21.0	33.0
15	27.6112	29.0	25.0	32.0	20.0	33.0
16	27.6638	29.0	25.0	32.0	20.0	33.0
17	27.5734	29.0	25.0	32.0	20.0	33.0
18	27.6348	29.0	25.0	32.0	20.0	33.0
19	27.6526	29.0	25.0	32.0	20.0	33.0
20	27.2716	29.0	24.0	32.0	19.0	33.0
21	27.2642	29.0	25.0	31.0	19.0	33.0
22	26.881	28.0	24.0	31.0	19.0	33.0
23	26.8124	28.0	24.0	31.0	19.0	33.0
24	26.8204	28.0	24.0	31.0	19.0	33.0
25	26.6606	28.0	24.0	31.0	18.0	33.0
26	26.3806	28.0	23.0	31.0	18.0	33.0
27	25.947	28.0	23.0	31.0	17.0	33.0
28	25.7446	27.0	22.0	31.0	16.0	33.0
29	25.6856	27.0	22.0	31.0	16.0	33.0
30	25.5228	27.0	22.0	31.0	16.0	33.0
31	25.354	27.0	22.0	30.0	16.0	32.0
32	25.1656	27.0	22.0	30.0	16.0	32.0
33	25.0164	26.0	21.0	30.0	15.0	32.0
34	24.75	26.0	21.0	30.0	15.0	32.0
35	24.3926	26.0	21.0	30.0	15.0	32.0
36	24.1122	25.0	20.0	30.0	14.0	32.0
37	23.8612	25.0	20.0	29.0	13.0	32.0
38	23.7544	25.0	20.0	29.0	13.0	32.0
39	23.485	25.0	19.0	29.0	13.0	32.0
40	23.1984	24.0	19.0	29.0	13.0	32.0
41	23.0976	24.0	19.0	29.0	13.0	32.0
42	22.9212	24.0	19.0	28.0	13.0	31.0
43	22.5172	24.0	18.0	28.0	12.0	31.0
44	22.3234	23.0	18.0	28.0	12.0	31.0
45	22.1372	23.0	18.0	28.0	11.0	31.0
46	22.2166	23.0	18.0	28.0	11.0	31.0
47	22.075	23.0	18.0	28.0	11.0	31.0
48	21.4592	22.0	17.0	27.0	11.0	31.0
49	21.0954	22.0	16.0	27.0	10.0	30.0
50	20.9356	22.0	16.0	27.0	10.0	30.0
51	20.6152	21.0	15.0	27.0	9.0	30.0
52	20.6588	21.0	16.0	27.0	9.0	30.0
53	19.5852	20.0	14.0	25.0	9.0	29.0
54	19.9424	21.0	14.0	26.0	7.0	30.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
8	4.0
9	4.0
10	2.0
11	2.0
12	4.0
13	6.0
14	4.0
15	11.0
16	20.0
17	50.0
18	98.0
19	149.0
20	210.0
21	275.0
22	367.0
23	425.0
24	518.0
25	540.0
26	539.0
27	532.0
28	468.0
29	360.0
30	234.0
31	122.0
32	51.0
33	5.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	1075	1632	1296	997
2	1202	1586	1327	885
3	1079	1443	1471	1007
4	1079	1414	1445	1062
5	1058	1486	1416	1040
6	1119	1462	1286	1133
7	1067	1516	1412	1005
8	1079	1453	1397	1071
9	1015	1424	1429	1132
10	1060	1542	1328	1070
11	1057	1432	1364	1147
12	1066	1444	1450	1040
13	1018	1461	1447	1074
14	1001	1460	1461	1078
15	1010	1461	1419	1110
16	1017	1469	1465	1049
17	1030	1457	1425	1088
18	1030	1396	1470	1104
19	1083	1423	1429	1065
20	1000	1462	1448	1090
21	1086	1425	1409	1080
22	1026	1544	1365	1065
23	1036	1445	1486	1033
24	1056	1431	1379	1134
25	1055	1453	1458	1034
26	1109	1419	1406	1064
27	1067	1426	1438	1068
28	967	1527	1373	1133
29	1045	1456	1452	1046
30	1055	1470	1372	1103
31	1042	1402	1433	1123
32	992	1418	1437	1151
33	1104	1431	1363	1101
34	1065	1464	1363	1107
35	1054	1471	1411	1063
36	1070	1433	1440	1056
37	1044	1471	1426	1057
38	1093	1451	1403	1052
39	1096	1412	1352	1138
40	1066	1425	1391	1118
41	1083	1405	1380	1129
42	1114	1401	1385	1097
43	1062	1421	1391	1125
44	1070	1449	1377	1103
45	1093	1464	1337	1106
46	1063	1377	1387	1173
47	1081	1413	1402	1102
48	1064	1422	1320	1194
49	1052	1452	1359	1136
50	1091	1407	1340	1160
51	1051	1386	1400	1161
52	1091	1507	1297	1104
53	1058	1416	1402	1123
54	1072	1345	1339	1221
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.44
2	41.74
3	41.72
4	42.82
5	41.959999999999994
6	45.04
7	41.44
8	43.0
9	42.94
10	42.6
11	44.080000000000005
12	42.120000000000005
13	41.839999999999996
14	41.58
15	42.4
16	41.32
17	42.36
18	42.68
19	42.96
20	41.8
21	43.32
22	41.82
23	41.38
24	43.8
25	41.78
26	43.47739095638256
27	42.70854170834167
28	42.0
29	41.82836567313463
30	43.16
31	43.3
32	42.87715086034414
33	44.10882176435287
34	43.44868973794759
35	42.34846969393879
36	42.528505701140226
37	42.03681472589035
38	42.90858171634327
39	44.697879151660665
40	43.68
41	44.266559935961574
42	44.24654792875725
43	43.74874974994999
44	43.468693738747746
45	43.980000000000004
46	44.72
47	43.677470988395356
48	45.16
49	43.76875375075015
50	45.038015206082434
51	44.25770308123249
52	43.908781756351274
53	43.628725745149026
54	46.071930882057465
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	5.0
2	7.0
3	7.0
4	7.0
5	5.0
6	2.0
7	1.0
8	1.5
9	2.0
10	1.5
11	1.0
12	2.5
13	4.0
14	4.0
15	4.0
16	5.0
17	6.0
18	11.0
19	19.5
20	23.0
21	37.5
22	52.0
23	61.0
24	70.0
25	79.0
26	88.0
27	117.5
28	147.0
29	159.5
30	172.0
31	203.0
32	243.0
33	252.0
34	290.0
35	328.0
36	330.0
37	332.0
38	332.5
39	333.0
40	342.0
41	351.0
42	343.5
43	336.0
44	318.5
45	279.5
46	258.0
47	254.5
48	251.0
49	234.5
50	218.0
51	209.5
52	201.0
53	198.5
54	196.0
55	177.0
56	162.5
57	167.0
58	147.0
59	127.0
60	106.5
61	86.0
62	85.0
63	84.0
64	61.5
65	39.0
66	42.5
67	46.0
68	34.0
69	17.0
70	12.0
71	14.0
72	16.0
73	12.5
74	9.0
75	6.5
76	4.0
77	4.5
78	5.0
79	5.0
80	5.0
81	3.0
82	1.0
83	1.0
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	1.0
90	2.0
91	3.0
92	2.5
93	2.0
94	2.0
95	3.0
96	4.0
97	5.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.04
27	0.02
28	0.0
29	0.02
30	0.0
31	0.0
32	0.04
33	0.02
34	0.02
35	0.02
36	0.02
37	0.04
38	0.02
39	0.04
40	0.0
41	0.06
42	0.06
43	0.02
44	0.02
45	0.0
46	0.0
47	0.04
48	0.0
49	0.02
50	0.04
51	0.04
52	0.02
53	0.02
54	0.45999999999999996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
54	5000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.66
#Duplication Level	Relative count
1	100.0
2	0.08053150795248641
3	0.040265753976243206
4	0.0
5	0.020132876988121603
6	0.0
7	0.040265753976243206
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT	7	0.13999999999999999	Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC	7	0.13999999999999999	Illumina Paired End PCR Primer 2 (100% over 35bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCCC	1110	9.557241	23.193876	44
AAAAA	2130	4.1300144	5.698024	7
CTCCC	530	3.5749238	11.56272	11
CCTCC	510	3.440021	11.56272	14
CTGGG	465	3.1575248	8.314476	45
TGGAA	785	2.929684	6.397646	17
GGGGG	335	2.9167063	10.660779	6
GAATG	775	2.8923633	10.053445	20
CCCAG	450	2.8820548	7.8420706	22
GTGTG	520	2.7661672	6.513526	23
TGTGT	660	2.744307	6.109576	27
GGGAG	425	2.7401958	6.315732	6
ATGGA	730	2.7244196	6.397646	20
GCTGG	395	2.6821985	6.651581	16
GGAGG	410	2.643483	7.894665	7
GGAAT	700	2.612457	7.3115954	19
GCAGG	400	2.5732677	14.17877	16
CCTGG	370	2.5068476	6.6367774	7
CAGCC	380	2.4337354	6.273657	4
TGGGA	480	2.419065	7.40506	20
AATGG	645	2.4071925	5.4836965	18
GCCTC	355	2.3998654	6.622006	2
CCCTC	355	2.394524	8.259085	13
CCAGC	370	2.3696895	6.2736564	14
GGAAG	495	2.3634295	14.031037	2
GGCTG	345	2.3426795	9.97737	24
CAGGA	490	2.3343499	13.99981	17
CCAGG	360	2.3107867	6.2876506	11
CTGTG	435	2.3088553	9.09864	44
GCCTG	340	2.3035896	8.295971	5
TCCCA	460	2.3028264	6.1297736	9
CTGCC	340	2.2984629	8.277508	10
CAGCA	480	2.2816207	10.47649	14
TTCCT	545	2.256056	7.0961456	1
CTCCT	415	2.1929073	6.4701333	15
TCCCC	325	2.1921701	6.6072683	28
CAGGC	335	2.1503155	6.2876506	14
CCCCA	335	2.1407545	6.259694	20
TGGTG	400	2.127821	6.513526	9
CCCCT	315	2.1247187	6.607268	12
GGCAG	330	2.122946	6.301676	16
CTCTG	400	2.1183603	5.187652	16
TTCCA	540	2.1177678	6.7228537	28
CAGAG	440	2.0961509	5.833255	28
CCCTG	310	2.095657	6.622006	24
AGGGA	435	2.0769534	5.846266	1
CTCAG	410	2.0570977	6.143447	24
CTTCC	385	2.034384	6.4701333	17
AGCAG	425	2.0246909	10.499857	15
AGGAA	570	2.015381	10.390455	18
TCCTC	380	2.0079634	5.1761065	1
TCAGC	400	2.0069244	12.286894	44
AGAGC	420	2.0008712	10.499858	5
TCCTG	375	1.9859626	6.4845653	8
TCTCC	375	1.9815428	5.1761065	8
CACCC	310	1.9809967	6.259694	9
TGTGG	370	1.9682345	5.210821	4
GATGG	390	1.9654902	6.1708827	1
TGGCT	370	1.9638538	9.098641	45
TCCCT	370	1.9551222	5.1761065	37
CAGGG	300	1.9299511	6.3016763	21
AAGAG	545	1.9269869	9.524584	4
CAGAA	545	1.9226985	6.0476103	6
CTGAG	380	1.9108309	6.15715	11
TCTCT	460	1.9041945	5.068676	8
GAGGC	295	1.897785	6.301676	22
CCCAC	295	1.885142	6.259694	4
CCTGC	275	1.8590509	9.93301	6
CCTGT	350	1.8535651	6.4845653	6
TTTCC	445	1.8421009	5.0686755	14
TGCCT	345	1.8270856	5.187652	12
CACAC	380	1.8022629	5.8073177	17
CCTCT	340	1.7965988	7.7641597	2
TTCCC	340	1.7965986	6.470133	18
GAAGA	500	1.767878	7.792842	26
CTGGC	260	1.7615684	6.636777	8
TTCAG	440	1.7294377	9.6255	12
CTCTC	325	1.717337	5.1761065	3
GTGGT	320	1.7022567	5.2108207	24
GGGTG	250	1.7013805	8.333022	28
TGCTT	410	1.7010024	5.0799813	4
CTTTG	405	1.6802584	5.0799813	2
AGAAG	475	1.6794841	5.1952276	22
ACTCC	330	1.6520276	6.1297736	10
CTGCT	310	1.6417291	6.4845653	3
GCCCC	190	1.6395733	6.339712	37
AGTGG	325	1.6379085	6.1708827	3
TGGCA	325	1.6342632	6.1571493	16
GGCTC	240	1.626063	6.636776	25
ACACA	460	1.6192161	5.1721287	14
GCTTT	390	1.6180266	5.0799813	5
TTCTG	390	1.6180266	5.0799813	3
TCCTT	390	1.6144258	6.0824113	16
CCCAA	335	1.588837	5.8073177	10
GCACA	330	1.5686142	5.8202715	18
GTTCA	395	1.5525635	7.700401	11
TCTTC	375	1.5523325	5.068676	4
AGGCA	325	1.5482932	5.8332543	6
GGGGA	240	1.5474048	6.3157325	7
CCTTC	290	1.5323931	6.4701333	17
ATGCC	305	1.5302798	12.286893	22
GAAGG	320	1.5278738	5.8462653	8
GGGAT	295	1.4867171	7.40506	21
TGTGC	280	1.4861598	5.199224	7
AACAG	420	1.4817125	5.1836653	10
TCTTT	455	1.4755235	5.5590916	1
TTGGC	275	1.4596211	5.199223	15
GAGAT	385	1.4368514	6.397646	27
TTTGG	345	1.4345239	6.1095753	3
GGTTC	270	1.4330826	10.398446	10
CCTTG	265	1.4034137	5.187652	18
CCCTT	265	1.4002901	5.176106	14
CTGTT	335	1.3898433	5.0799813	10
CCTTT	335	1.3867503	5.068676	8
GGCAC	215	1.3800533	6.2876506	17
TTGAA	465	1.3564935	6.4295163	7
CTTGG	255	1.3534669	5.1992235	18
GTGTT	325	1.3513632	5.091313	28
GGCCC	155	1.3405302	6.353853	2
TGGTT	320	1.330573	5.091313	25
TTCTT	410	1.3295923	7.941558	4
GGGGT	195	1.327077	8.333022	45
AAGGA	375	1.3259085	5.1952276	44
CAGAT	350	1.3033215	5.471493	10
CTGGT	245	1.3003898	5.1992235	12
TGTCC	245	1.2974956	5.187652	21
GCTCC	190	1.2844352	8.277508	26
GACAG	265	1.2624544	5.833254	27
GAGCG	195	1.2544682	12.603353	6
TTGGG	235	1.2500948	6.5135255	6
AATGC	335	1.2474647	10.031068	21
GGCTT	235	1.2473125	5.199223	4
TTTGC	295	1.223892	5.0799813	9
GGTGT	230	1.223497	5.21082	27
GATCG	235	1.181698	6.1571493	45
AGCTC	225	1.1288949	6.1434464	25
ACCCT	225	1.1263824	6.129773	10
CGGAA	235	1.119535	9.333206	1
CCCAT	220	1.1013516	6.129773	21
TCTTG	265	1.0994283	5.0799813	21
ATGCA	295	1.0985136	5.4714913	37
GCTTG	205	1.0880812	5.199223	2
AGCGG	165	1.061473	12.603352	7
AACCA	300	1.0560105	5.1721287	22
AACCT	280	1.0403365	5.4593143	13
CCGAG	160	1.0270164	12.575301	25
TTGCC	190	1.0062209	5.1876516	3
ACTCA	265	0.9846043	5.4593153	11
CTCAT	250	0.98044825	5.7624474	4
GTTCC	185	0.9797416	6.4845653	27
GAACC	205	0.97444224	5.820272	25
GGCGG	110	0.9555928	10.637051	44
GCGGT	130	0.8827489	13.303162	8
GCGGG	100	0.86872077	6.3822303	45
TGCCG	125	0.846908	14.93275	23
AGGCG	120	0.7719804	6.3016763	15
CGGTT	140	0.74307984	10.398446	9
GCCGA	115	0.738168	12.575301	24
CGAGA	130	0.61931723	9.333206	26
CGGGA	85	0.5468194	6.301676	24
CACGG	85	0.54560244	6.2876506	37
>>END_MODULE