/tools/next_gen_conversion/solid2fastq.xml
https://bitbucket.org/cistrome/cistrome-harvard/ · XML · 154 lines · 127 code · 27 blank · 0 comment · 0 complexity · 8e3b93323d16a5250b0d8f19c13b820e MD5 · raw file
- <tool id="solid2fastq" name="Convert">
- <description>SOLiD output to fastq</description>
- <command interpreter="python">
- #if $is_run.paired == "no" #solid2fastq.py --fr=$input1 --fq=$input2 --fout=$out_file1 -q $qual $trim_name $trim_first_base $double_encode
- #elif $is_run.paired == "yes" #solid2fastq.py --fr=$input1 --fq=$input2 --fout=$out_file1 --rr=$input3 --rq=$input4 --rout=$out_file2 -q $qual $trim_name $trim_first_base $double_encode
- #end if#
- </command>
- <inputs>
- <param name="input1" type="data" format="csfasta" label="Select reads"/>
- <param name="input2" type="data" format="qualsolid" label="Select qualities"/>
- <conditional name="is_run">
- <param name="paired" type="select" label="Is this a mate-pair run?">
- <option value="no" selected="true">No</option>
- <option value="yes">Yes</option>
- </param>
- <when value="yes">
- <param name="input3" type="data" format="csfasta" label="Select Reverse reads"/>
- <param name="input4" type="data" format="qualsolid" label="Select Reverse qualities"/>
- </when>
- <when value="no">
- </when>
- </conditional>
- <param name="qual" label="Remove reads containing color qualities below this value" type="integer" value="0"/>
- <param name="trim_name" type="select" label="Trim trailing "_F3" and "_R3" ?">
- <option value="-t" selected="true">Yes</option>
- <option value="">No</option>
- </param>
- <param name="trim_first_base" type="select" label="Trim first base?">
- <option value="-f">Yes (BWA)</option>
- <option value="" selected="true">No (bowtie)</option>
- </param>
- <param name="double_encode" type="select" label="Double encode?">
- <option value="-d">Yes (BWA)</option>
- <option value="" selected="true">No (bowtie)</option>
- </param>
- </inputs>
- <outputs>
- <data format="fastqcssanger" name="out_file1"/>
- <data format="fastqcssanger" name="out_file2">
- <filter>is_run['paired'] == 'yes'</filter>
- </data>
- </outputs>
- <tests>
- <test>
- <param name="input1" value="fr.csfasta" ftype="csfasta"/>
- <param name="input2" value="fr.qualsolid" ftype="qualsolid" />
- <param name="paired" value="no"/>
- <param name="qual" value="0" />
- <param name="trim_first_base" value="No" />
- <param name="trim_name" value="No" />
- <param name="double_encode" value="No"/>
- <output name="out_file1" file="solid2fastq_out_1.fastq"/>
- </test>
- <test>
- <param name="input1" value="fr.csfasta" ftype="csfasta"/>
- <param name="input2" value="fr.qualsolid" ftype="qualsolid" />
- <param name="paired" value="yes"/>
- <param name="input3" value="rr.csfasta" ftype="csfasta"/>
- <param name="input4" value="rr.qualsolid" ftype="qualsolid" />
- <param name="qual" value="0" />
- <param name="trim_first_base" value="No" />
- <param name="trim_name" value="Yes" />
- <param name="double_encode" value="No"/>
- <output name="out_file1" file="solid2fastq_out_2.fastq"/>
- <output name="out_file2" file="solid2fastq_out_3.fastq"/>
- </test>
- </tests>
- <help>
- **What it does**
- Converts output of SOLiD instrument (versions 3.5 and earlier) to fastq format suitable for bowtie, bwa, and PerM mappers.
- --------
- **Input datasets**
- Below are examples of forward (F3) reads and quality scores:
- Reads::
- >1831_573_1004_F3
- T00030133312212111300011021310132222
- >1831_573_1567_F3
- T03330322230322112131010221102122113
- Quality scores::
- >1831_573_1004_F3
- 4 29 34 34 32 32 24 24 20 17 10 34 29 20 34 13 30 34 22 24 11 28 19 17 34 17 24 17 25 34 7 24 14 12 22
- >1831_573_1567_F3
- 8 26 31 31 16 22 30 31 28 29 22 30 30 31 32 23 30 28 28 31 19 32 30 32 19 8 32 10 13 6 32 10 6 16 11
- **Mate pairs**
- If your data is from a mate-paired run, you will have additional read and quality datasets that will look similar to the ones above with one exception: the names of reads will be ending with "_R3".
- In this case choose **Yes** from the *Is this a mate-pair run?* drop down and you will be able to select R reads. When processing mate pairs this tool generates two output files: one for F3 reads and the other for R3 reads.
- The reads are guaranteed to be paired -- mated reads will be in the same position in F3 and R3 fastq file. However, because pairing is verified it may take a while to process an entire SOLiD run (several hours).
- ------
- **Explanation of parameters**
- **Remove reads containing color qualities below this value** - any read that contains as least one color call with quality lower than the specified value **will not** be reported.
- **Trim trailing "_F3" and "_R3"?** - does just that. Not necessary for bowtie. Required for BWA.
- **Trim first base?** - SOLiD reads contain an adapter base such as the first T in this read::
- >1831_573_1004_F3
- T00030133312212111300011021310132222
-
- this option removes this base leaving only color calls. Not necessary for bowtie. Required for BWA.
- **Double encode?** - converts color calls (0123.) to pseudo-nucleotides (ACGTN). Not necessary for bowtie. Required for BWA.
- ------
- **Examples of output**
- When all parameters are left "as-is" you will get this (using reads and qualities shown above)::
- @1831_573_1004
- T00030133312212111300011021310132222
- +
- %%>CCAA9952+C>5C.?C79,=42C292:C(9/-7
- @1831_573_1004
- T03330322230322112131010221102122113
- +
- );@@17?@=>7??@A8?==@4A?A4)A+.'A+'1,
- Setting *Trim first base from reads* to **Yes** will produce this::
- @1831_573_1004
- 00030133312212111300011021310132222
- +
- %%>CCAA9952+C>5C.?C79,=42C292:C(9/-7
- @1831_573_1004
- 03330322230322112131010221102122113
- +
- );@@17?@=>7??@A8?==@4A?A4)A+.'A+'1,
- Finally, setting *Double encode* to **Yes** will yield::
- @1831_573_1004
- TAAATACTTTCGGCGCCCTAAACCAGCTCACTGGGG
- +
- %%>CCAA9952+C>5C.?C79,=42C292:C(9/-7
- @1831_573_1004
- TATTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT
- +
- );@@17?@=>7??@A8?==@4A?A4)A+.'A+'1,
- </help>
- </tool>