<tool id="EMBOSS: dan19" name="dan" version="5.0.0">

  <description>Calculates DNA RNA/DNA melting temperature</description>

  <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements>

  <command interpreter="perl">emboss_single_outputfile_wrapper.pl dan -sequence $input1 -windowsize $window -goutfile $out_file1 -graph png -plot $plot1 -shiftincrement $shift -dnaconc $dnaconc

  -saltconc $saltconc -product $product -formamide $formamide -mismatch $mismatch -prodlen $prodlen -thermo $thermo -temperature $temperature -rna $rna -outfile $out_file1 -auto</command>

  <inputs>

    <param format="data" name="input1" type="data">

      <label>On query</label>

    </param>

    <param name="window" size="4" type="text" value="20">

      <label>Window Size</label>

    </param>

    <param name="shift" size="4" type="text" value="1">

      <label>Step size (shift increment)</label>

    </param>

    <param name="dnaconc" size="4" type="text" value="50.0">

      <label>DNA Concentration (nM)</label>

    </param>

    <param name="saltconc" size="4" type="text" value="50.0">

      <label>Salt concentration (mM)</label>

    </param>

    <param name="thermo" type="select">

      <label>Output the DeltaG, DeltaH and DeltaS values</label>

      <option value="yes">Yes</option>

      <option value="no">No</option>

    </param>

    <param name="temperature" size="4" type="text" value="25 ">

      <label>Temperature at which to calculate the DeltaG, DeltaH and DeltaS values</label>

    </param>

    <param name="rna" type="select">

      <label>Sequence is RNA</label>

      <option value="no">No</option>

      <option value="yes">Yes</option>

    </param>

    <param name="product" type="select">

      <label>Include percent formamide, percent of mismatches allowed and product length</label>

      <option value="no">No</option>

      <option value="yes">Yes</option>

    </param>

    <param name="formamide" size="4" type="text" value="0 ">

      <label>Formamide concentration (nM)</label>

    </param>

    <param name="mismatch" size="4" type="text" value="0 ">

      <label>Percent mismatch to be used in calculations</label>

    </param>

    <param name="prodlen" size="4" type="text" value="20">

      <label>Product length to be used in calculations</label>

    </param>

    <param name="plot1" type="select">

      <label>Create a graph</label>

      <option value="no">No</option>

      <option value="yes">Yes</option>

    </param>

  </inputs>

  <outputs>

    <data format="dan" name="out_file1" />

  </outputs>

  <!--
  <tests>
    <test>
      <param name="input1" value="2.fasta"/>
      <param name="window" value="20"/>
      <param name="shift" value="1"/>
      <param name="dnaconc" value="50"/>
      <param name="saltconc" value="50"/>
      <param name="thermo" value="yes"/>
      <param name="temperature" value="25"/>
      <param name="rna" value="no"/>
      <param name="product" value="no"/>
      <param name="formamide" value="0"/>
      <param name="mismatch" value="0"/>
      <param name="prodlen" value="20"/>
      <param name="plot1" value="yes"/>
      <output name="out_file1" file="emboss_dan_out.png"/>
    </test>
  </tests>

  -->

  <code file="emboss_format_corrector.py" />

  <help>
    You can view the original documentation here_.
    
    .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/dan.html



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**Citation**



If you use this tool in Galaxy, please cite `Blankenberg D, Taylor J, Schenck I, He J, Zhang Y, Ghent M, Veeraraghavan N, Albert I, Miller W, Makova KD, Hardison RC, Nekrutenko A. A framework for collaborative analysis of ENCODE data: making large-scale analyses biologist-friendly. Genome Res. 2007 Jun;17(6):960-4. &lt;http://www.ncbi.nlm.nih.gov/pubmed/17568012&gt;`_
  </help>
</tool>