/tools/genetrack/genetrack_indexer.xml

 1<tool id="bed2genetrack" name="GeneTrack indexer" version="1.0.1">
 2  
 3  <description>on a BED file</description>
 4
 5  <command interpreter="python">
 6    genetrack_indexer.py -i $input -o $output -s $shift -v 0 -f BED -x
 7  </command>
 8    
 9  <inputs>
10    
11    <param format="bed6" name="input" type="data" help="Input data">
12      <label>Select input bed file</label>
13    </param>
14    
15    <param name="shift" size="4" type="integer" value="0" help="distance in basepairs">
16        <label>Shift at 5' end</label>
17    </param>
18
19    <!-- this parameter is currently not used, may not be feasible to use it
20    <param name="coverage" type="select" label="Full coverage">
21      <option value="no">NO</option>
22      <option value="yes">YES</option>
23    </param>
24    -->
25  
26  </inputs>
27
28  <outputs>  
29    <data format="genetrack" name="output" />
30  </outputs>
31   
32<help>
33**Help**
34
35This tool will create a visualization of the bed file that is selected. 
36
37**Parameters**
38
39- **Shift at 5' end** should be used when the location of interest is at a fixed distance from
40  the 5' end for **all sequenced fragments**! 
41  
42  For example if the sequenced sample consists
43  mono-nucleosomal DNA (146bp) we should expect that 
44  each nucleosome midpoint is located at 73 bp from the 5' end of the fragment. 
45  Therefore we would enter 73 as the shift parameter. Once corrected the reads 
46  on each strand will coincide and indicate the actual midpoints 
47  of the nucleosomes.
48  
49  When shifting the averaging process in GeneTrack is able correct for longer or shorter
50  than expected fragment sizes as long as the errors are reasonably random.
51
52See http://genetrack.bx.psu.edu/ for more information on GeneTrack.
53
54------
55
56**Citation**
57
58For the underlying tool, please cite `Albert I, Wachi S, Jiang C, Pugh BF. GeneTrack--a genomic data processing and visualization framework. Bioinformatics. 2008 May 15;24(10):1305-6. &lt;http://www.ncbi.nlm.nih.gov/pubmed/18388141&gt;`_
59
60If you use this tool in Galaxy, please cite Blankenberg D, et al. *In preparation.*
61
62</help>
63
64</tool>