/tools/metag_tools/short_reads_trim_seq.xml
https://bitbucket.org/ialbert/galaxy-genetrack · XML · 93 lines · 69 code · 24 blank · 0 comment · 0 complexity · 1d67bb2d47265220dc29cc04ab2115d2 MD5 · raw file
- <tool id="trim_reads" name="Select high quality segments" version="1.0.0">
- <description>from short reads</description>
- <command interpreter="python">
- short_reads_trim_seq.py $trim $length $output1 $input1 $input2 $sequencing_method_choice.input3
- </command>
- <inputs>
- <page>
- <param name="input1" type="data" format="fasta,txtseq.zip" label="Reads" />
- <param name="input2" type="data" format="qualsolexa,qual454,txtseq.zip" label="Quality scores" />
- <param name="trim" type="integer" size="5" value="20" label="Minimal quality score" help="bases scoring below this value will trigger splitting"/>
- <param name="length" type="integer" size="5" value="100" label="Minimal length of contiguous segment" help="report all high quality segments above this length. Setting this option to '0' will cause the program to return a single longest run of high quality bases per read" />
- <conditional name="sequencing_method_choice">
- <param name="sequencer" type="select" label="Select technology">
- <option value="454">Roche (454) or ABI SOLiD</option>
- <option value="Solexa">Illumina (Solexa)</option>
- </param>
- <when value="454">
- <param name="input3" type="select" label="Low quality bases in homopolymers" help="if set to 'DO NOT trigger splitting' the program will not count low quality bases that are within or adjacent to homonucleotide runs. This will significantly reduce fragmentation of 454 data">
- <option value="yes">DO NOT trigger splitting </option>
- <option value="no">trigger splitting</option>
- </param>
- </when>
- <when value="Solexa">
- <param name="input3" type="integer" size="5" value="0" label="Restrict length of each read to" help="('0' = do not trim) The quality of Solexa reads drops towards the end. This option allows selecting the specified number of nucleotides from the beginning and then running the tool." />
- </when>
- </conditional>
- </page>
- </inputs>
- <outputs>
- <data name="output1" format="fasta" />
- </outputs>
- <tests>
- <test>
- <param name="sequencer" value="454" />
- <param name="input1" value="454.fasta" ftype="fasta" />
- <param name="input2" value="454.qual" ftype="qual454" />
- <param name="input3" value="no" />
- <param name="trim" value="20" />
- <param name="length" value="0" />
- <output name="output1" file="short_reads_trim_seq_out1.fasta" />
- </test>
- <test>
- <param name="sequencer" value="Solexa" />
- <param name="input1" value="solexa.fasta" ftype="fasta" />
- <param name="input2" value="solexa.qual" ftype="qualsolexa" />
- <param name="input3" value="0" />
- <param name="trim" value="20" />
- <param name="length" value="0" />
- <output name="output1" file="short_reads_trim_seq_out2.fasta" />
- </test>
- </tests>
- <help>
-
- .. class:: warningmark
- To use this tool your quality score dataset needs to be in *Quality Score* format. Click pencil icon next to your dataset to set datatype to *Quality Score*.
-
- -----
- **What it does**
- This tool finds high quality segments within sequencing reads generated by by Roche (454), Illumina (Solexa), or ABI SOLiD machines.
- -----
- **Example**
- Suppose this is your sequencing read::
-
- 5'---------*-------------*------**----3'
-
- where **dashes** (-) are HIGH quality bases (above 20) and **asterisks** (*) are LOW quality bases (below 20). If the **Minimal length of contiguous segment** is set to **5** (of course, only for the purposes of this example), the tool will return::
- 5'---------
- -------------
- -------
- you can see that the tool simply splits the read on low quality bases and then returns all segments longer than 5. **Note**, that the output of this tool will likely contain higher number of shorter sequences compared to the original input. If we set the **Minimal length of contiguous segment** to **0**, the tool will only return the single longest segment::
- -------------
-
-
- </help>
- </tool>