/test/test_project_utils.rb

https://github.com/holroyd/martsearch · Ruby · 852 lines · 845 code · 6 blank · 1 comment · 0 complexity · a7cdd61deee05e0d634cd49fec24cdbf MD5 · raw file 

    

  1. # encoding: utf-8
    2. 

  2. 

  3. require "test_helper"
    4. 

  4. 

  5. class TestMartSearchProjectUtils < Test::Unit::TestCase
    6. 

  6.   include MartSearch::ProjectUtils
    7. 

  7.   public  :get_top_level_project_info, :get_human_orthalog, :get_mice, :get_mutagenesis_predictions
    8. 

  8.   
    9. 

  9.   context "A valid MartSearch Project" do
    10. 

  10.     setup do
    11. 

  11.       VCR.insert_cassette( "test_project_utils" )
    12. 

  12.       @datasources = MartSearch::Controller.instance().datasources
    13. 

  13.       @project_id  = 35505
    14. 

  14.     end
    15. 

  15. 

  16.     should "have top level information" do
    17. 

  17.       expected = {
    18. 

  18.         :marker_symbol    => "Cbx1",
    19. 

  19.         :mgi_accession_id => "MGI:105369",
    20. 

  20.         :ensembl_gene_id  => "ENSMUSG00000018666",
    21. 

  21.         :vega_gene_id     => "OTTMUSG00000001636",
    22. 

  22.         :ikmc_project     => "EUCOMM",
    23. 

  23.         :status           => "Mice - Genotype confirmed",
    24. 

  24.         :mouse_available  => "1",
    25. 

  25.         :escell_available => "1",
    26. 

  26.         :vector_available => "1"
    27. 

  27.       }
    28. 

  28.       assert_equal( expected, get_top_level_project_info( @datasources, @project_id )[:data][0] )
    29. 

  29.     end
    30. 

  30. 

  31.     should "have the correct human orthalog" do
    32. 

  32.       human_orth_data = get_human_orthalog( @datasources, "ENSMUSG00000018666" )[:data]
    33. 

  33.       assert_equal( "ENSG00000108468", human_orth_data[:human_ensembl_gene] )
    34. 

  34.     end
    35. 

  35. 

  36.     should "have the expected results" do
    37. 

  37.       expected_int_vectors = [
    38. 

  38.         {
    39. 

  39.           :name               => "PCS00019_A_B11",
    40. 

  40.           :design_id          => "39792",
    41. 

  41.           :design_type        => "Knockout-First - Reporter Tagged Insertion"
    42. 

  42.         }
    43. 

  43.       ]
    44. 

  44.       expected_targ_vectors = [
    45. 

  45.         {
    46. 

  46.           :name           => "PG00019_A_1_B11",
    47. 

  47.           :design_id      => "39792",
    48. 

  48.           :design_type    => "Knockout-First - Reporter Tagged Insertion",
    49. 

  49.           :cassette       => "L1L2_gt2",
    50. 

  50.           :cassette_type  => "Promotorless",
    51. 

  51.           :backbone       => "L3L4_pZero_kan",
    52. 

  52.         },
    53. 

  53.         {
    54. 

  54.           :name           => "PG00019_A_2_B11",
    55. 

  55.           :design_id      => "39792",
    56. 

  56.           :design_type    => "Knockout-First - Reporter Tagged Insertion",
    57. 

  57.           :cassette       => "L1L2_gt2",
    58. 

  58.           :cassette_type  => "Promotorless",
    59. 

  59.           :backbone       => "L3L4_pZero_kan"
    60. 

  60.         },
    61. 

  61.         {
    62. 

  62.           :name           => "PG00019_A_3_B11",
    63. 

  63.           :design_id      => "39792",
    64. 

  64.           :design_type    => "Knockout-First - Reporter Tagged Insertion",
    65. 

  65.           :cassette       => "L1L2_gt2",
    66. 

  66.           :cassette_type  => "Promotorless",
    67. 

  67.           :backbone       => "L3L4_pZero_kan"
    68. 

  68.         },
    69. 

  69.         {
    70. 

  70.           :name           => "PG00019_A_4_B11",
    71. 

  71.           :design_id      => "39792",
    72. 

  72.           :design_type    => "Knockout-First - Reporter Tagged Insertion",
    73. 

  73.           :cassette       => "L1L2_gt2",
    74. 

  74.           :cassette_type  => "Promotorless",
    75. 

  75.           :backbone       => "L3L4_pZero_kan"
    76. 

  76.         },
    77. 

  77.         {
    78. 

  78.           :name           => "PGS00019_A_B11",
    79. 

  79.           :design_id      => "39792",
    80. 

  80.           :design_type    => "Knockout-First - Reporter Tagged Insertion",
    81. 

  81.           :cassette       => "L1L2_gt2",
    82. 

  82.           :cassette_type  => "Promotorless",
    83. 

  83.           :backbone       => "L3L4_pZero_kan"
    84. 

  84.         }
    85. 

  85.       ]
    86. 

  86.       expected_cells = {
    87. 

  87.         :conditional => {
    88. 

  88.           :cells => [
    89. 

  89.             {
    90. 

  90.               :name                                  => "EPD0027_2_B01",
    91. 

  91.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    92. 

  92.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    93. 

  93.               :parental_cell_line                    => "JM8.N4",
    94. 

  94.               :targeting_vector                      => "PGS00019_A_B11",
    95. 

  95.               :cassette                              => "L1L2_gt2",
    96. 

  96.               :cassette_type                         => "Promotorless",
    97. 

  97.               :"mouse?"                              => "yes",
    98. 

  98.               :qc_count                              => 3,
    99. 

  99.               :production_qc_five_prime_screen       => "pass",
    100. 

  100.               :production_qc_loxp_screen             => "pass",
    101. 

  101.               :production_qc_three_prime_screen      => "pass",
    102. 

  102.               :production_qc_loss_of_allele          => "-",
    103. 

  103.               :production_qc_vector_integrity        => "-",
    104. 

  104.               :distribution_qc_karyotype_high        => "-",
    105. 

  105.               :distribution_qc_karyotype_low         => "-",
    106. 

  106.               :distribution_qc_copy_number           => "-",
    107. 

  107.               :distribution_qc_five_prime_lr_pcr     => "-",
    108. 

  108.               :distribution_qc_five_prime_sr_pcr     => "-",
    109. 

  109.               :distribution_qc_three_prime_sr_pcr    => "-",
    110. 

  110.               :distribution_qc_thawing               => "-",
    111. 

  111.               :user_qc_southern_blot                 => "-",
    112. 

  112.               :user_qc_map_test                      => "-",
    113. 

  113.               :user_qc_karyotype                     => "-",
    114. 

  114.               :user_qc_tv_backbone_assay             => "-",
    115. 

  115.               :user_qc_five_prime_lr_pcr             => "-",
    116. 

  116.               :user_qc_loss_of_wt_allele             => "-",
    117. 

  117.               :user_qc_neo_count_qpcr                => "-",
    118. 

  118.               :user_qc_lacz_sr_pcr                   => "-",
    119. 

  119.               :user_qc_five_prime_cassette_integrity => "-",
    120. 

  120.               :user_qc_neo_sr_pcr                    => "-",
    121. 

  121.               :user_qc_mutant_specific_sr_pcr        => "-",
    122. 

  122.               :user_qc_loxp_confirmation             => "-",
    123. 

  123.               :user_qc_three_prime_lr_pcr            => "-"
    124. 

  124.             },
    125. 

  125.             {
    126. 

  126.               :name                                  => "EPD0027_2_A02",
    127. 

  127.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    128. 

  128.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    129. 

  129.               :parental_cell_line                    => "JM8.N4",
    130. 

  130.               :targeting_vector                      => "PGS00019_A_B11",
    131. 

  131.               :cassette                              => "L1L2_gt2",
    132. 

  132.               :cassette_type                         => "Promotorless",
    133. 

  133.               :"mouse?"                              => "no",
    134. 

  134.               :qc_count                              => 3,
    135. 

  135.               :production_qc_five_prime_screen       => "pass",
    136. 

  136.               :production_qc_loxp_screen             => "pass",
    137. 

  137.               :production_qc_three_prime_screen      => "pass",
    138. 

  138.               :production_qc_loss_of_allele          => "-",
    139. 

  139.               :production_qc_vector_integrity        => "-",
    140. 

  140.               :distribution_qc_karyotype_high        => "-",
    141. 

  141.               :distribution_qc_karyotype_low         => "-",
    142. 

  142.               :distribution_qc_copy_number           => "-",
    143. 

  143.               :distribution_qc_five_prime_lr_pcr     => "-",
    144. 

  144.               :distribution_qc_five_prime_sr_pcr     => "-",
    145. 

  145.               :distribution_qc_three_prime_sr_pcr    => "-",
    146. 

  146.               :distribution_qc_thawing               => "-",
    147. 

  147.               :user_qc_southern_blot                 => "-",
    148. 

  148.               :user_qc_map_test                      => "-",
    149. 

  149.               :user_qc_karyotype                     => "-",
    150. 

  150.               :user_qc_tv_backbone_assay             => "-",
    151. 

  151.               :user_qc_five_prime_lr_pcr             => "-",
    152. 

  152.               :user_qc_loss_of_wt_allele             => "-",
    153. 

  153.               :user_qc_neo_count_qpcr                => "-",
    154. 

  154.               :user_qc_lacz_sr_pcr                   => "-",
    155. 

  155.               :user_qc_five_prime_cassette_integrity => "-",
    156. 

  156.               :user_qc_neo_sr_pcr                    => "-",
    157. 

  157.               :user_qc_mutant_specific_sr_pcr        => "-",
    158. 

  158.               :user_qc_loxp_confirmation             => "-",
    159. 

  159.               :user_qc_three_prime_lr_pcr            => "-"
    160. 

  160.             },
    161. 

  161.             {
    162. 

  162.               :name                                  => "EPD0027_2_B03",
    163. 

  163.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    164. 

  164.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    165. 

  165.               :parental_cell_line                    => "JM8.N4",
    166. 

  166.               :targeting_vector                      => "PGS00019_A_B11",
    167. 

  167.               :cassette                              => "L1L2_gt2",
    168. 

  168.               :cassette_type                         => "Promotorless",
    169. 

  169.               :"mouse?"                              => "no",
    170. 

  170.               :qc_count                              => 3,
    171. 

  171.               :production_qc_five_prime_screen       => "pass",
    172. 

  172.               :production_qc_loxp_screen             => "pass",
    173. 

  173.               :production_qc_three_prime_screen      => "pass",
    174. 

  174.               :production_qc_loss_of_allele          => "-",
    175. 

  175.               :production_qc_vector_integrity        => "-",
    176. 

  176.               :distribution_qc_karyotype_high        => "-",
    177. 

  177.               :distribution_qc_karyotype_low         => "-",
    178. 

  178.               :distribution_qc_copy_number           => "-",
    179. 

  179.               :distribution_qc_five_prime_lr_pcr     => "-",
    180. 

  180.               :distribution_qc_five_prime_sr_pcr     => "-",
    181. 

  181.               :distribution_qc_three_prime_sr_pcr    => "-",
    182. 

  182.               :distribution_qc_thawing               => "-",
    183. 

  183.               :user_qc_southern_blot                 => "-",
    184. 

  184.               :user_qc_map_test                      => "-",
    185. 

  185.               :user_qc_karyotype                     => "-",
    186. 

  186.               :user_qc_tv_backbone_assay             => "-",
    187. 

  187.               :user_qc_five_prime_lr_pcr             => "-",
    188. 

  188.               :user_qc_loss_of_wt_allele             => "-",
    189. 

  189.               :user_qc_neo_count_qpcr                => "-",
    190. 

  190.               :user_qc_lacz_sr_pcr                   => "-",
    191. 

  191.               :user_qc_five_prime_cassette_integrity => "-",
    192. 

  192.               :user_qc_neo_sr_pcr                    => "-",
    193. 

  193.               :user_qc_mutant_specific_sr_pcr        => "-",
    194. 

  194.               :user_qc_loxp_confirmation             => "-",
    195. 

  195.               :user_qc_three_prime_lr_pcr            => "-"
    196. 

  196.             },
    197. 

  197.             {
    198. 

  198.               :name                                  => "EPD0027_2_C01",
    199. 

  199.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    200. 

  200.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    201. 

  201.               :parental_cell_line                    => "JM8.N4",
    202. 

  202.               :targeting_vector                      => "PGS00019_A_B11",
    203. 

  203.               :cassette                              => "L1L2_gt2",
    204. 

  204.               :cassette_type                         => "Promotorless",
    205. 

  205.               :"mouse?"                              => "no",
    206. 

  206.               :qc_count                              => 3,
    207. 

  207.               :production_qc_five_prime_screen       => "pass",
    208. 

  208.               :production_qc_loxp_screen             => "pass",
    209. 

  209.               :production_qc_three_prime_screen      => "pass",
    210. 

  210.               :production_qc_loss_of_allele          => "-",
    211. 

  211.               :production_qc_vector_integrity        => "-",
    212. 

  212.               :distribution_qc_karyotype_high        => "-",
    213. 

  213.               :distribution_qc_karyotype_low         => "-",
    214. 

  214.               :distribution_qc_copy_number           => "-",
    215. 

  215.               :distribution_qc_five_prime_lr_pcr     => "-",
    216. 

  216.               :distribution_qc_five_prime_sr_pcr     => "-",
    217. 

  217.               :distribution_qc_three_prime_sr_pcr    => "-",
    218. 

  218.               :distribution_qc_thawing               => "-",
    219. 

  219.               :user_qc_southern_blot                 => "-",
    220. 

  220.               :user_qc_map_test                      => "-",
    221. 

  221.               :user_qc_karyotype                     => "-",
    222. 

  222.               :user_qc_tv_backbone_assay             => "-",
    223. 

  223.               :user_qc_five_prime_lr_pcr             => "-",
    224. 

  224.               :user_qc_loss_of_wt_allele             => "-",
    225. 

  225.               :user_qc_neo_count_qpcr                => "-",
    226. 

  226.               :user_qc_lacz_sr_pcr                   => "-",
    227. 

  227.               :user_qc_five_prime_cassette_integrity => "-",
    228. 

  228.               :user_qc_neo_sr_pcr                    => "-",
    229. 

  229.               :user_qc_mutant_specific_sr_pcr        => "-",
    230. 

  230.               :user_qc_loxp_confirmation             => "-",
    231. 

  231.               :user_qc_three_prime_lr_pcr            => "-"
    232. 

  232.             },
    233. 

  233.             {
    234. 

  234.               :name                                  => "EPD0027_2_C03",
    235. 

  235.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    236. 

  236.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    237. 

  237.               :parental_cell_line                    => "JM8.N4",
    238. 

  238.               :targeting_vector                      => "PGS00019_A_B11",
    239. 

  239.               :cassette                              => "L1L2_gt2",
    240. 

  240.               :cassette_type                         => "Promotorless",
    241. 

  241.               :"mouse?"                              => "no",
    242. 

  242.               :qc_count                              => 3,
    243. 

  243.               :production_qc_five_prime_screen       => "pass",
    244. 

  244.               :production_qc_loxp_screen             => "pass",
    245. 

  245.               :production_qc_three_prime_screen      => "pass",
    246. 

  246.               :production_qc_loss_of_allele          => "-",
    247. 

  247.               :production_qc_vector_integrity        => "-",
    248. 

  248.               :distribution_qc_karyotype_high        => "-",
    249. 

  249.               :distribution_qc_karyotype_low         => "-",
    250. 

  250.               :distribution_qc_copy_number           => "-",
    251. 

  251.               :distribution_qc_five_prime_lr_pcr     => "-",
    252. 

  252.               :distribution_qc_five_prime_sr_pcr     => "-",
    253. 

  253.               :distribution_qc_three_prime_sr_pcr    => "-",
    254. 

  254.               :distribution_qc_thawing               => "-",
    255. 

  255.               :user_qc_southern_blot                 => "-",
    256. 

  256.               :user_qc_map_test                      => "-",
    257. 

  257.               :user_qc_karyotype                     => "-",
    258. 

  258.               :user_qc_tv_backbone_assay             => "-",
    259. 

  259.               :user_qc_five_prime_lr_pcr             => "-",
    260. 

  260.               :user_qc_loss_of_wt_allele             => "-",
    261. 

  261.               :user_qc_neo_count_qpcr                => "-",
    262. 

  262.               :user_qc_lacz_sr_pcr                   => "-",
    263. 

  263.               :user_qc_five_prime_cassette_integrity => "-",
    264. 

  264.               :user_qc_neo_sr_pcr                    => "-",
    265. 

  265.               :user_qc_mutant_specific_sr_pcr        => "-",
    266. 

  266.               :user_qc_loxp_confirmation             => "-",
    267. 

  267.               :user_qc_three_prime_lr_pcr            => "-"
    268. 

  268.             },
    269. 

  269.             {
    270. 

  270.               :name                                  => "EPD0027_2_E01",
    271. 

  271.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    272. 

  272.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    273. 

  273.               :parental_cell_line                    => "JM8.N4",
    274. 

  274.               :targeting_vector                      => "PGS00019_A_B11",
    275. 

  275.               :cassette                              => "L1L2_gt2",
    276. 

  276.               :cassette_type                         => "Promotorless",
    277. 

  277.               :"mouse?"                              => "no",
    278. 

  278.               :qc_count                              => 3,
    279. 

  279.               :production_qc_five_prime_screen       => "pass",
    280. 

  280.               :production_qc_loxp_screen             => "pass",
    281. 

  281.               :production_qc_three_prime_screen      => "pass",
    282. 

  282.               :production_qc_loss_of_allele          => "-",
    283. 

  283.               :production_qc_vector_integrity        => "-",
    284. 

  284.               :distribution_qc_karyotype_high        => "-",
    285. 

  285.               :distribution_qc_karyotype_low         => "-",
    286. 

  286.               :distribution_qc_copy_number           => "-",
    287. 

  287.               :distribution_qc_five_prime_lr_pcr     => "-",
    288. 

  288.               :distribution_qc_five_prime_sr_pcr     => "-",
    289. 

  289.               :distribution_qc_three_prime_sr_pcr    => "-",
    290. 

  290.               :distribution_qc_thawing               => "-",
    291. 

  291.               :user_qc_southern_blot                 => "-",
    292. 

  292.               :user_qc_map_test                      => "-",
    293. 

  293.               :user_qc_karyotype                     => "-",
    294. 

  294.               :user_qc_tv_backbone_assay             => "-",
    295. 

  295.               :user_qc_five_prime_lr_pcr             => "-",
    296. 

  296.               :user_qc_loss_of_wt_allele             => "-",
    297. 

  297.               :user_qc_neo_count_qpcr                => "-",
    298. 

  298.               :user_qc_lacz_sr_pcr                   => "-",
    299. 

  299.               :user_qc_five_prime_cassette_integrity => "-",
    300. 

  300.               :user_qc_neo_sr_pcr                    => "-",
    301. 

  301.               :user_qc_mutant_specific_sr_pcr        => "-",
    302. 

  302.               :user_qc_loxp_confirmation             => "-",
    303. 

  303.               :user_qc_three_prime_lr_pcr            => "-"
    304. 

  304.             },
    305. 

  305.             {
    306. 

  306.               :name                                  => "EPD0027_2_E02",
    307. 

  307.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    308. 

  308.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    309. 

  309.               :parental_cell_line                    => "JM8.N4",
    310. 

  310.               :targeting_vector                      => "PGS00019_A_B11",
    311. 

  311.               :cassette                              => "L1L2_gt2",
    312. 

  312.               :cassette_type                         => "Promotorless",
    313. 

  313.               :"mouse?"                              => "no",
    314. 

  314.               :qc_count                              => 3,
    315. 

  315.               :production_qc_five_prime_screen       => "pass",
    316. 

  316.               :production_qc_loxp_screen             => "pass",
    317. 

  317.               :production_qc_three_prime_screen      => "pass",
    318. 

  318.               :production_qc_loss_of_allele          => "-",
    319. 

  319.               :production_qc_vector_integrity        => "-",
    320. 

  320.               :distribution_qc_karyotype_high        => "-",
    321. 

  321.               :distribution_qc_karyotype_low         => "-",
    322. 

  322.               :distribution_qc_copy_number           => "-",
    323. 

  323.               :distribution_qc_five_prime_lr_pcr     => "-",
    324. 

  324.               :distribution_qc_five_prime_sr_pcr     => "-",
    325. 

  325.               :distribution_qc_three_prime_sr_pcr    => "-",
    326. 

  326.               :distribution_qc_thawing               => "-",
    327. 

  327.               :user_qc_southern_blot                 => "-",
    328. 

  328.               :user_qc_map_test                      => "-",
    329. 

  329.               :user_qc_karyotype                     => "-",
    330. 

  330.               :user_qc_tv_backbone_assay             => "-",
    331. 

  331.               :user_qc_five_prime_lr_pcr             => "-",
    332. 

  332.               :user_qc_loss_of_wt_allele             => "-",
    333. 

  333.               :user_qc_neo_count_qpcr                => "-",
    334. 

  334.               :user_qc_lacz_sr_pcr                   => "-",
    335. 

  335.               :user_qc_five_prime_cassette_integrity => "-",
    336. 

  336.               :user_qc_neo_sr_pcr                    => "-",
    337. 

  337.               :user_qc_mutant_specific_sr_pcr        => "-",
    338. 

  338.               :user_qc_loxp_confirmation             => "-",
    339. 

  339.               :user_qc_three_prime_lr_pcr            => "-"
    340. 

  340.             },
    341. 

  341.             {
    342. 

  342.               :name                                  => "EPD0027_2_F01",
    343. 

  343.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    344. 

  344.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    345. 

  345.               :parental_cell_line                    => "JM8.N4",
    346. 

  346.               :targeting_vector                      => "PGS00019_A_B11",
    347. 

  347.               :cassette                              => "L1L2_gt2",
    348. 

  348.               :cassette_type                         => "Promotorless",
    349. 

  349.               :"mouse?"                              => "no",
    350. 

  350.               :qc_count                              => 3,
    351. 

  351.               :production_qc_five_prime_screen       => "pass",
    352. 

  352.               :production_qc_loxp_screen             => "pass",
    353. 

  353.               :production_qc_three_prime_screen      => "pass",
    354. 

  354.               :production_qc_loss_of_allele          => "-",
    355. 

  355.               :production_qc_vector_integrity        => "-",
    356. 

  356.               :distribution_qc_karyotype_high        => "-",
    357. 

  357.               :distribution_qc_karyotype_low         => "-",
    358. 

  358.               :distribution_qc_copy_number           => "-",
    359. 

  359.               :distribution_qc_five_prime_lr_pcr     => "-",
    360. 

  360.               :distribution_qc_five_prime_sr_pcr     => "-",
    361. 

  361.               :distribution_qc_three_prime_sr_pcr    => "-",
    362. 

  362.               :distribution_qc_thawing               => "-",
    363. 

  363.               :user_qc_southern_blot                 => "-",
    364. 

  364.               :user_qc_map_test                      => "-",
    365. 

  365.               :user_qc_karyotype                     => "-",
    366. 

  366.               :user_qc_tv_backbone_assay             => "-",
    367. 

  367.               :user_qc_five_prime_lr_pcr             => "-",
    368. 

  368.               :user_qc_loss_of_wt_allele             => "-",
    369. 

  369.               :user_qc_neo_count_qpcr                => "-",
    370. 

  370.               :user_qc_lacz_sr_pcr                   => "-",
    371. 

  371.               :user_qc_five_prime_cassette_integrity => "-",
    372. 

  372.               :user_qc_neo_sr_pcr                    => "-",
    373. 

  373.               :user_qc_mutant_specific_sr_pcr        => "-",
    374. 

  374.               :user_qc_loxp_confirmation             => "-",
    375. 

  375.               :user_qc_three_prime_lr_pcr            => "-"
    376. 

  376.             },
    377. 

  377.             {
    378. 

  378.               :name                                  => "EPD0027_2_F02",
    379. 

  379.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    380. 

  380.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    381. 

  381.               :parental_cell_line                    => "JM8.N4",
    382. 

  382.               :targeting_vector                      => "PGS00019_A_B11",
    383. 

  383.               :cassette                              => "L1L2_gt2",
    384. 

  384.               :cassette_type                         => "Promotorless",
    385. 

  385.               :"mouse?"                              => "no",
    386. 

  386.               :qc_count                              => 3,
    387. 

  387.               :production_qc_five_prime_screen       => "pass",
    388. 

  388.               :production_qc_loxp_screen             => "pass",
    389. 

  389.               :production_qc_three_prime_screen      => "pass",
    390. 

  390.               :production_qc_loss_of_allele          => "-",
    391. 

  391.               :production_qc_vector_integrity        => "-",
    392. 

  392.               :distribution_qc_karyotype_high        => "-",
    393. 

  393.               :distribution_qc_karyotype_low         => "-",
    394. 

  394.               :distribution_qc_copy_number           => "-",
    395. 

  395.               :distribution_qc_five_prime_lr_pcr     => "-",
    396. 

  396.               :distribution_qc_five_prime_sr_pcr     => "-",
    397. 

  397.               :distribution_qc_three_prime_sr_pcr    => "-",
    398. 

  398.               :distribution_qc_thawing               => "-",
    399. 

  399.               :user_qc_southern_blot                 => "-",
    400. 

  400.               :user_qc_map_test                      => "-",
    401. 

  401.               :user_qc_karyotype                     => "-",
    402. 

  402.               :user_qc_tv_backbone_assay             => "-",
    403. 

  403.               :user_qc_five_prime_lr_pcr             => "-",
    404. 

  404.               :user_qc_loss_of_wt_allele             => "-",
    405. 

  405.               :user_qc_neo_count_qpcr                => "-",
    406. 

  406.               :user_qc_lacz_sr_pcr                   => "-",
    407. 

  407.               :user_qc_five_prime_cassette_integrity => "-",
    408. 

  408.               :user_qc_neo_sr_pcr                    => "-",
    409. 

  409.               :user_qc_mutant_specific_sr_pcr        => "-",
    410. 

  410.               :user_qc_loxp_confirmation             => "-",
    411. 

  411.               :user_qc_three_prime_lr_pcr            => "-"
    412. 

  412.             },
    413. 

  413.             {
    414. 

  414.               :name                                  => "EPD0027_2_F03",
    415. 

  415.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    416. 

  416.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    417. 

  417.               :parental_cell_line                    => "JM8.N4",
    418. 

  418.               :targeting_vector                      => "PGS00019_A_B11",
    419. 

  419.               :cassette                              => "L1L2_gt2",
    420. 

  420.               :cassette_type                         => "Promotorless",
    421. 

  421.               :"mouse?"                              => "no",
    422. 

  422.               :qc_count                              => 3,
    423. 

  423.               :production_qc_five_prime_screen       => "pass",
    424. 

  424.               :production_qc_loxp_screen             => "pass",
    425. 

  425.               :production_qc_three_prime_screen      => "pass",
    426. 

  426.               :production_qc_loss_of_allele          => "-",
    427. 

  427.               :production_qc_vector_integrity        => "-",
    428. 

  428.               :distribution_qc_karyotype_high        => "-",
    429. 

  429.               :distribution_qc_karyotype_low         => "-",
    430. 

  430.               :distribution_qc_copy_number           => "-",
    431. 

  431.               :distribution_qc_five_prime_lr_pcr     => "-",
    432. 

  432.               :distribution_qc_five_prime_sr_pcr     => "-",
    433. 

  433.               :distribution_qc_three_prime_sr_pcr    => "-",
    434. 

  434.               :distribution_qc_thawing               => "-",
    435. 

  435.               :user_qc_southern_blot                 => "-",
    436. 

  436.               :user_qc_map_test                      => "-",
    437. 

  437.               :user_qc_karyotype                     => "-",
    438. 

  438.               :user_qc_tv_backbone_assay             => "-",
    439. 

  439.               :user_qc_five_prime_lr_pcr             => "-",
    440. 

  440.               :user_qc_loss_of_wt_allele             => "-",
    441. 

  441.               :user_qc_neo_count_qpcr                => "-",
    442. 

  442.               :user_qc_lacz_sr_pcr                   => "-",
    443. 

  443.               :user_qc_five_prime_cassette_integrity => "-",
    444. 

  444.               :user_qc_neo_sr_pcr                    => "-",
    445. 

  445.               :user_qc_mutant_specific_sr_pcr        => "-",
    446. 

  446.               :user_qc_loxp_confirmation             => "-",
    447. 

  447.               :user_qc_three_prime_lr_pcr            => "-"
    448. 

  448.             },
    449. 

  449.             {
    450. 

  450.               :name                                  => "EPD0027_2_A01",
    451. 

  451.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    452. 

  452.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    453. 

  453.               :parental_cell_line                    => "JM8.N4",
    454. 

  454.               :targeting_vector                      => "PGS00019_A_B11",
    455. 

  455.               :cassette                              => "L1L2_gt2",
    456. 

  456.               :cassette_type                         => "Promotorless",
    457. 

  457.               :"mouse?"                              => "no",
    458. 

  458.               :qc_count                              => 4,
    459. 

  459.               :production_qc_five_prime_screen       => "pass",
    460. 

  460.               :production_qc_loxp_screen             => "pass",
    461. 

  461.               :production_qc_three_prime_screen      => "pass",
    462. 

  462.               :production_qc_loss_of_allele          => "-",
    463. 

  463.               :production_qc_vector_integrity        => "-",
    464. 

  464.               :distribution_qc_karyotype_high        => "-",
    465. 

  465.               :distribution_qc_karyotype_low         => "-",
    466. 

  466.               :distribution_qc_copy_number           => "-",
    467. 

  467.               :distribution_qc_five_prime_lr_pcr     => "-",
    468. 

  468.               :distribution_qc_five_prime_sr_pcr     => "-",
    469. 

  469.               :distribution_qc_three_prime_sr_pcr    => "fail",
    470. 

  470.               :distribution_qc_thawing               => "-",
    471. 

  471.               :user_qc_southern_blot                 => "-",
    472. 

  472.               :user_qc_map_test                      => "-",
    473. 

  473.               :user_qc_karyotype                     => "-",
    474. 

  474.               :user_qc_tv_backbone_assay             => "-",
    475. 

  475.               :user_qc_five_prime_lr_pcr             => "-",
    476. 

  476.               :user_qc_loss_of_wt_allele             => "-",
    477. 

  477.               :user_qc_neo_count_qpcr                => "-",
    478. 

  478.               :user_qc_lacz_sr_pcr                   => "-",
    479. 

  479.               :user_qc_five_prime_cassette_integrity => "-",
    480. 

  480.               :user_qc_neo_sr_pcr                    => "-",
    481. 

  481.               :user_qc_mutant_specific_sr_pcr        => "-",
    482. 

  482.               :user_qc_loxp_confirmation             => "-",
    483. 

  483.               :user_qc_three_prime_lr_pcr            => "-"
    484. 

  484.             },
    485. 

  485.             {
    486. 

  486.               :name                                  => "EPD0027_2_H01",
    487. 

  487.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    488. 

  488.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    489. 

  489.               :parental_cell_line                    => "JM8.N4",
    490. 

  490.               :targeting_vector                      => "PGS00019_A_B11",
    491. 

  491.               :cassette                              => "L1L2_gt2",
    492. 

  492.               :cassette_type                         => "Promotorless",
    493. 

  493.               :"mouse?"                              => "no",
    494. 

  494.               :qc_count                              => 5,
    495. 

  495.               :production_qc_five_prime_screen       => "pass",
    496. 

  496.               :production_qc_loxp_screen             => "pass",
    497. 

  497.               :production_qc_three_prime_screen      => "pass",
    498. 

  498.               :production_qc_loss_of_allele          => "-",
    499. 

  499.               :production_qc_vector_integrity        => "-",
    500. 

  500.               :distribution_qc_karyotype_high        => "-",
    501. 

  501.               :distribution_qc_karyotype_low         => "-",
    502. 

  502.               :distribution_qc_copy_number           => "-",
    503. 

  503.               :distribution_qc_five_prime_lr_pcr     => "-",
    504. 

  504.               :distribution_qc_five_prime_sr_pcr     => "pass",
    505. 

  505.               :distribution_qc_three_prime_sr_pcr    => "pass",
    506. 

  506.               :distribution_qc_thawing               => "-",
    507. 

  507.               :user_qc_southern_blot                 => "-",
    508. 

  508.               :user_qc_map_test                      => "-",
    509. 

  509.               :user_qc_karyotype                     => "-",
    510. 

  510.               :user_qc_tv_backbone_assay             => "-",
    511. 

  511.               :user_qc_five_prime_lr_pcr             => "-",
    512. 

  512.               :user_qc_loss_of_wt_allele             => "-",
    513. 

  513.               :user_qc_neo_count_qpcr                => "-",
    514. 

  514.               :user_qc_lacz_sr_pcr                   => "-",
    515. 

  515.               :user_qc_five_prime_cassette_integrity => "-",
    516. 

  516.               :user_qc_neo_sr_pcr                    => "-",
    517. 

  517.               :user_qc_mutant_specific_sr_pcr        => "-",
    518. 

  518.               :user_qc_loxp_confirmation             => "-",
    519. 

  519.               :user_qc_three_prime_lr_pcr            => "-"
    520. 

  520.             },
    521. 

  521.             {
    522. 

  522.               :name                                  => "EPD0027_2_H02",
    523. 

  523.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    524. 

  524.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    525. 

  525.               :parental_cell_line                    => "JM8.N4",
    526. 

  526.               :targeting_vector                      => "PGS00019_A_B11",
    527. 

  527.               :cassette                              => "L1L2_gt2",
    528. 

  528.               :cassette_type                         => "Promotorless",
    529. 

  529.               :"mouse?"                              => "no",
    530. 

  530.               :qc_count                              => 5,
    531. 

  531.               :production_qc_five_prime_screen       => "pass",
    532. 

  532.               :production_qc_loxp_screen             => "pass",
    533. 

  533.               :production_qc_three_prime_screen      => "pass",
    534. 

  534.               :production_qc_loss_of_allele          => "-",
    535. 

  535.               :production_qc_vector_integrity        => "-",
    536. 

  536.               :distribution_qc_karyotype_high        => "-",
    537. 

  537.               :distribution_qc_karyotype_low         => "-",
    538. 

  538.               :distribution_qc_copy_number           => "-",
    539. 

  539.               :distribution_qc_five_prime_lr_pcr     => "-",
    540. 

  540.               :distribution_qc_five_prime_sr_pcr     => "pass",
    541. 

  541.               :distribution_qc_three_prime_sr_pcr    => "pass",
    542. 

  542.               :distribution_qc_thawing               => "-",
    543. 

  543.               :user_qc_southern_blot                 => "-",
    544. 

  544.               :user_qc_map_test                      => "-",
    545. 

  545.               :user_qc_karyotype                     => "-",
    546. 

  546.               :user_qc_tv_backbone_assay             => "-",
    547. 

  547.               :user_qc_five_prime_lr_pcr             => "-",
    548. 

  548.               :user_qc_loss_of_wt_allele             => "-",
    549. 

  549.               :user_qc_neo_count_qpcr                => "-",
    550. 

  550.               :user_qc_lacz_sr_pcr                   => "-",
    551. 

  551.               :user_qc_five_prime_cassette_integrity => "-",
    552. 

  552.               :user_qc_neo_sr_pcr                    => "-",
    553. 

  553.               :user_qc_mutant_specific_sr_pcr        => "-",
    554. 

  554.               :user_qc_loxp_confirmation             => "-",
    555. 

  555.               :user_qc_three_prime_lr_pcr            => "-"
    556. 

  556.             },
    557. 

  557.             {
    558. 

  558.               :name                                  => "EPD0027_2_H03",
    559. 

  559.               :allele_symbol_superscript             => "tm1a(EUCOMM)Wtsi",
    560. 

  560.               :allele_type                           => "Knockout-First - Reporter Tagged Insertion",
    561. 

  561.               :parental_cell_line                    => "JM8.N4",
    562. 

  562.               :targeting_vector                      => "PGS00019_A_B11",
    563. 

  563.               :cassette                              => "L1L2_gt2",
    564. 

  564.               :cassette_type                         => "Promotorless",
    565. 

  565.               :"mouse?"                              => "no",
    566. 

  566.               :qc_count                              => 5,
    567. 

  567.               :production_qc_five_prime_screen       => "pass",
    568. 

  568.               :production_qc_loxp_screen             => "pass",
    569. 

  569.               :production_qc_three_prime_screen      => "pass",
    570. 

  570.               :production_qc_loss_of_allele          => "-",
    571. 

  571.               :production_qc_vector_integrity        => "-",
    572. 

  572.               :distribution_qc_karyotype_high        => "-",
    573. 

  573.               :distribution_qc_karyotype_low         => "-",
    574. 

  574.               :distribution_qc_copy_number           => "-",
    575. 

  575.               :distribution_qc_five_prime_lr_pcr     => "-",
    576. 

  576.               :distribution_qc_five_prime_sr_pcr     => "pass",
    577. 

  577.               :distribution_qc_three_prime_sr_pcr    => "pass",
    578. 

  578.               :distribution_qc_thawing               => "-",
    579. 

  579.               :user_qc_southern_blot                 => "-",
    580. 

  580.               :user_qc_map_test                      => "-",
    581. 

  581.               :user_qc_karyotype                     => "-",
    582. 

  582.               :user_qc_tv_backbone_assay             => "-",
    583. 

  583.               :user_qc_five_prime_lr_pcr             => "-",
    584. 

  584.               :user_qc_loss_of_wt_allele             => "-",
    585. 

  585.               :user_qc_neo_count_qpcr                => "-",
    586. 

  586.               :user_qc_lacz_sr_pcr                   => "-",
    587. 

  587.               :user_qc_five_prime_cassette_integrity => "-",
    588. 

  588.               :user_qc_neo_sr_pcr                    => "-",
    589. 

  589.               :user_qc_mutant_specific_sr_pcr        => "-",
    590. 

  590.               :user_qc_loxp_confirmation             => "-",
    591. 

  591.               :user_qc_three_prime_lr_pcr            => "-"
    592. 

  592.             }
    593. 

  593.           ],
    594. 

  594.           :allele_img  => "http://www.knockoutmouse.org/targ_rep/alleles/902/allele-image",
    595. 

  595.           :allele_gb   => "http://www.knockoutmouse.org/targ_rep/alleles/902/escell-clone-genbank-file",
    596. 

  596.         },
    597. 

  597.         :"targeted non-conditional" => {
    598. 

  598.           :cells => [
    599. 

  599.             {
    600. 

  600.               :name                                  => "EPD0027_2_A03",
    601. 

  601.               :allele_symbol_superscript             => "tm1e(EUCOMM)Wtsi",
    602. 

  602.               :allele_type                           => "Targeted Non-Conditional",
    603. 

  603.               :parental_cell_line                    => "JM8.N4",
    604. 

  604.               :targeting_vector                      => "PGS00019_A_B11",
    605. 

  605.               :cassette                              => "L1L2_gt2",
    606. 

  606.               :cassette_type                         => "Promotorless",
    607. 

  607.               :"mouse?"                              => "no",
    608. 

  608.               :qc_count                              => 3,
    609. 

  609.               :production_qc_five_prime_screen       => "pass",
    610. 

  610.               :production_qc_loxp_screen             => "not confirmed",
    611. 

  611.               :production_qc_three_prime_screen      => "pass",
    612. 

  612.               :production_qc_loss_of_allele          => "-",
    613. 

  613.               :production_qc_vector_integrity        => "-",
    614. 

  614.               :distribution_qc_karyotype_high        => "-",
    615. 

  615.               :distribution_qc_karyotype_low         => "-",
    616. 

  616.               :distribution_qc_copy_number           => "-",
    617. 

  617.               :distribution_qc_five_prime_lr_pcr     => "-",
    618. 

  618.               :distribution_qc_five_prime_sr_pcr     => "-",
    619. 

  619.               :distribution_qc_three_prime_sr_pcr    => "-",
    620. 

  620.               :distribution_qc_thawing               => "-",
    621. 

  621.               :user_qc_southern_blot                 => "-",
    622. 

  622.               :user_qc_map_test                      => "-",
    623. 

  623.               :user_qc_karyotype                     => "-",
    624. 

  624.               :user_qc_tv_backbone_assay             => "-",
    625. 

  625.               :user_qc_five_prime_lr_pcr             => "-",
    626. 

  626.               :user_qc_loss_of_wt_allele             => "-",
    627. 

  627.               :user_qc_neo_count_qpcr                => "-",
    628. 

  628.               :user_qc_lacz_sr_pcr                   => "-",
    629. 

  629.               :user_qc_five_prime_cassette_integrity => "-",
    630. 

  630.               :user_qc_neo_sr_pcr                    => "-",
    631. 

  631.               :user_qc_mutant_specific_sr_pcr        => "-",
    632. 

  632.               :user_qc_loxp_confirmation             => "-",
    633. 

  633.               :user_qc_three_prime_lr_pcr            => "-"
    634. 

  634.             },
    635. 

  635.             {
    636. 

  636.               :name                                  => "EPD0027_2_B02",
    637. 

  637.               :allele_symbol_superscript             => "tm1e(EUCOMM)Wtsi",
    638. 

  638.               :allele_type                           => "Targeted Non-Conditional",
    639. 

  639.               :parental_cell_line                    => "JM8.N4",
    640. 

  640.               :targeting_vector                      => "PGS00019_A_B11",
    641. 

  641.               :cassette                              => "L1L2_gt2",
    642. 

  642.               :cassette_type                         => "Promotorless",
    643. 

  643.               :"mouse?"                              => "no",
    644. 

  644.               :qc_count                              => 3,
    645. 

  645.               :production_qc_five_prime_screen       => "pass",
    646. 

  646.               :production_qc_loxp_screen             => "not confirmed",
    647. 

  647.               :production_qc_three_prime_screen      => "pass",
    648. 

  648.               :production_qc_loss_of_allele          => "-",
    649. 

  649.               :production_qc_vector_integrity        => "-",
    650. 

  650.               :distribution_qc_karyotype_high        => "-",
    651. 

  651.               :distribution_qc_karyotype_low         => "-",
    652. 

  652.               :distribution_qc_copy_number           => "-",
    653. 

  653.               :distribution_qc_five_prime_lr_pcr     => "-",
    654. 

  654.               :distribution_qc_five_prime_sr_pcr     => "-",
    655. 

  655.               :distribution_qc_three_prime_sr_pcr    => "-",
    656. 

  656.               :distribution_qc_thawing               => "-",
    657. 

  657.               :user_qc_southern_blot                 => "-",
    658. 

  658.               :user_qc_map_test                      => "-",
    659. 

  659.               :user_qc_karyotype                     => "-",
    660. 

  660.               :user_qc_tv_backbone_assay             => "-",
    661. 

  661.               :user_qc_five_prime_lr_pcr             => "-",
    662. 

  662.               :user_qc_loss_of_wt_allele             => "-",
    663. 

  663.               :user_qc_neo_count_qpcr                => "-",
    664. 

  664.               :user_qc_lacz_sr_pcr                   => "-",
    665. 

  665.               :user_qc_five_prime_cassette_integrity => "-",
    666. 

  666.               :user_qc_neo_sr_pcr                    => "-",
    667. 

  667.               :user_qc_mutant_specific_sr_pcr        => "-",
    668. 

  668.               :user_qc_loxp_confirmation             => "-",
    669. 

  669.               :user_qc_three_prime_lr_pcr            => "-"
    670. 

  670.             },
    671. 

  671.             {
    672. 

  672.               :name                                  => "EPD0027_2_D01",
    673. 

  673.               :allele_symbol_superscript             => "tm1e(EUCOMM)Wtsi",
    674. 

  674.               :allele_type                           => "Targeted Non-Conditional",
    675. 

  675.               :parental_cell_line                    => "JM8.N4",
    676. 

  676.               :targeting_vector                      => "PGS00019_A_B11",
    677. 

  677.               :cassette                              => "L1L2_gt2",
    678. 

  678.               :cassette_type                         => "Promotorless",
    679. 

  679.               :"mouse?"                              => "no",
    680. 

  680.               :qc_count                              => 3,
    681. 

  681.               :production_qc_five_prime_screen       => "pass",
    682. 

  682.               :production_qc_loxp_screen             => "not confirmed",
    683. 

  683.               :production_qc_three_prime_screen      => "not confirmed",
    684. 

  684.               :production_qc_loss_of_allele          => "-",
    685. 

  685.               :production_qc_vector_integrity        => "-",
    686. 

  686.               :distribution_qc_karyotype_high        => "-",
    687. 

  687.               :distribution_qc_karyotype_low         => "-",
    688. 

  688.               :distribution_qc_copy_number           => "-",
    689. 

  689.               :distribution_qc_five_prime_lr_pcr     => "-",
    690. 

  690.               :distribution_qc_five_prime_sr_pcr     => "-",
    691. 

  691.               :distribution_qc_three_prime_sr_pcr    => "-",
    692. 

  692.               :distribution_qc_thawing               => "-",
    693. 

  693.               :user_qc_southern_blot                 => "-",
    694. 

  694.               :user_qc_map_test                      => "-",
    695. 

  695.               :user_qc_karyotype                     => "-",
    696. 

  696.               :user_qc_tv_backbone_assay             => "-",
    697. 

  697.               :user_qc_five_prime_lr_pcr             => "-",
    698. 

  698.               :user_qc_loss_of_wt_allele             => "-",
    699. 

  699.               :user_qc_neo_count_qpcr                => "-",
    700. 

  700.               :user_qc_lacz_sr_pcr                   => "-",
    701. 

  701.               :user_qc_five_prime_cassette_integrity => "-",
    702. 

  702.               :user_qc_neo_sr_pcr                    => "-",
    703. 

  703.               :user_qc_mutant_specific_sr_pcr        => "-",
    704. 

  704.               :user_qc_loxp_confirmation             => "-",
    705. 

  705.               :user_qc_three_prime_lr_pcr            => "-"
    706. 

  706.             },
    707. 

  707.             {
    708. 

  708.               :name                                  => "EPD0027_2_D02",
    709. 

  709.               :allele_symbol_superscript             => "tm1e(EUCOMM)Wtsi",
    710. 

  710.               :allele_type                           => "Targeted Non-Conditional",
    711. 

  711.               :parental_cell_line                    => "JM8.N4",
    712. 

  712.               :targeting_vector                      => "PGS00019_A_B11",
    713. 

  713.               :cassette                              => "L1L2_gt2",
    714. 

  714.               :cassette_type                         => "Promotorless",
    715. 

  715.               :"mouse?"                              => "no",
    716. 

  716.               :qc_count                              => 3,
    717. 

  717.               :production_qc_five_prime_screen       => "pass",
    718. 

  718.               :production_qc_loxp_screen             => "not confirmed",
    719. 

  719.               :production_qc_three_prime_screen      => "not confirmed",
    720. 

  720.               :production_qc_loss_of_allele          => "-",
    721. 

  721.               :production_qc_vector_integrity        => "-",
    722. 

  722.               :distribution_qc_karyotype_high        => "-",
    723. 

  723.               :distribution_qc_karyotype_low         => "-",
    724. 

  724.               :distribution_qc_copy_number           => "-",
    725. 

  725.               :distribution_qc_five_prime_lr_pcr     => "-",
    726. 

  726.               :distribution_qc_five_prime_sr_pcr     => "-",
    727. 

  727.               :distribution_qc_three_prime_sr_pcr    => "-",
    728. 

  728.               :distribution_qc_thawing               => "-",
    729. 

  729.               :user_qc_southern_blot                 => "-",
    730. 

  730.               :user_qc_map_test                      => "-",
    731. 

  731.               :user_qc_karyotype                     => "-",
    732. 

  732.               :user_qc_tv_backbone_assay             => "-",
    733. 

  733.               :user_qc_five_prime_lr_pcr             => "-",
    734. 

  734.               :user_qc_loss_of_wt_allele             => "-",
    735. 

  735.               :user_qc_neo_count_qpcr                => "-",
    736. 

  736.               :user_qc_lacz_sr_pcr                   => "-",
    737. 

  737.               :user_qc_five_prime_cassette_integrity => "-",
    738. 

  738.               :user_qc_neo_sr_pcr                    => "-",
    739. 

  739.               :user_qc_mutant_specific_sr_pcr        => "-",
    740. 

  740.               :user_qc_loxp_confirmation             => "-",
    741. 

  741.               :user_qc_three_prime_lr_pcr            => "-"
    742. 

  742.             },
    743. 

  743.             {
    744. 

  744.               :name                                  => "EPD0027_2_D03",
    745. 

  745.               :allele_symbol_superscript             => "tm1e(EUCOMM)Wtsi",
    746. 

  746.               :allele_type                           => "Targeted Non-Conditional",
    747. 

  747.               :parental_cell_line                    => "JM8.N4",
    748. 

  748.               :targeting_vector                      => "PGS00019_A_B11",
    749. 

  749.               :cassette                              => "L1L2_gt2",
    750. 

  750.               :cassette_type                         => "Promotorless",
    751. 

  751.               :"mouse?"                              => "no",
    752. 

  752.               :qc_count                              => 3,
    753. 

  753.               :production_qc_five_prime_screen       => "pass",
    754. 

  754.               :production_qc_loxp_screen             => "not confirmed",
    755. 

  755.               :production_qc_three_prime_screen      => "not confirmed",
    756. 

  756.               :production_qc_loss_of_allele          => "-",
    757. 

  757.               :production_qc_vector_integrity        => "-",
    758. 

  758.               :distribution_qc_karyotype_high        => "-",
    759. 

  759.               :distribution_qc_karyotype_low         => "-",
    760. 

  760.               :distribution_qc_copy_number           => "-",
    761. 

  761.               :distribution_qc_five_prime_lr_pcr     => "-",
    762. 

  762.               :distribution_qc_five_prime_sr_pcr     => "-",
    763. 

  763.               :distribution_qc_three_prime_sr_pcr    => "-",
    764. 

  764.               :distribution_qc_thawing               => "-",
    765. 

  765.               :user_qc_southern_blot                 => "-",
    766. 

  766.               :user_qc_map_test                      => "-",
    767. 

  767.               :user_qc_karyotype                     => "-",
    768. 

  768.               :user_qc_tv_backbone_assay             => "-",
    769. 

  769.               :user_qc_five_prime_lr_pcr             => "-",
    770. 

  770.               :user_qc_loss_of_wt_allele             => "-",
    771. 

  771.               :user_qc_neo_count_qpcr                => "-",
    772. 

  772.               :user_qc_lacz_sr_pcr                   => "-",
    773. 

  773.               :user_qc_five_prime_cassette_integrity => "-",
    774. 

  774.               :user_qc_neo_sr_pcr                    => "-",
    775. 

  775.               :user_qc_mutant_specific_sr_pcr        => "-",
    776. 

  776.               :user_qc_loxp_confirmation             => "-",
    777. 

  777.               :user_qc_three_prime_lr_pcr            => "-"
    778. 

  778.             },
    779. 

  779.             {
    780. 

  780.               :name                                  => "EPD0027_2_E03",
    781. 

  781.               :allele_symbol_superscript             => "tm1e(EUCOMM)Wtsi",
    782. 

  782.               :allele_type                           => "Targeted Non-Conditional",
    783. 

  783.               :parental_cell_line                    => "JM8.N4",
    784. 

  784.               :targeting_vector                      => "PGS00019_A_B11",
    785. 

  785.               :cassette                              => "L1L2_gt2",
    786. 

  786.               :cassette_type                         => "Promotorless",
    787. 

  787.               :"mouse?"                              => "no",
    788. 

  788.               :qc_count                              => 3,
    789. 

  789.               :production_qc_five_prime_screen       => "pass",
    790. 

  790.               :production_qc_loxp_screen             => "not confirmed",
    791. 

  791.               :production_qc_three_prime_screen      => "pass",
    792. 

  792.               :production_qc_loss_of_allele          => "-",
    793. 

  793.               :production_qc_vector_integrity        => "-",
    794. 

  794.               :distribution_qc_karyotype_high        => "-",
    795. 

  795.               :distribution_qc_karyotype_low         => "-",
    796. 

  796.               :distribution_qc_copy_number           => "-",
    797. 

  797.               :distribution_qc_five_prime_lr_pcr     => "-",
    798. 

  798.               :distribution_qc_five_prime_sr_pcr     => "-",
    799. 

  799.               :distribution_qc_three_prime_sr_pcr    => "-",
    800. 

  800.               :distribution_qc_thawing               => "-",
    801. 

  801.               :user_qc_southern_blot                 => "-",
    802. 

  802.               :user_qc_map_test                      => "-",
    803. 

  803.               :user_qc_karyotype                     => "-",
    804. 

  804.               :user_qc_tv_backbone_assay             => "-",
    805. 

  805.               :user_qc_five_prime_lr_pcr             => "-",
    806. 

  806.               :user_qc_loss_of_wt_allele             => "-",
    807. 

  807.               :user_qc_neo_count_qpcr                => "-",
    808. 

  808.               :user_qc_lacz_sr_pcr                   => "-",
    809. 

  809.               :user_qc_five_prime_cassette_integrity => "-",
    810. 

  810.               :user_qc_neo_sr_pcr                    => "-",
    811. 

  811.               :user_qc_mutant_specific_sr_pcr        => "-",
    812. 

  812.               :user_qc_loxp_confirmation             => "-",
    813. 

  813.               :user_qc_three_prime_lr_pcr            => "-"
    814. 

  814.             },
    815. 

  815.             {
    816. 

  816.               :name                                  => "EPD0027_2_G01",
    817. 

  817.               :allele_symbol_superscript             => "tm1e(EUCOMM)Wtsi",
    818. 

  818.               :allele_type                           => "Targeted Non-Conditional",
    819. 

  819.               :parental_cell_line                    => "JM8.N4",
    820. 

  820.               :targeting_vector                      => "PGS00019_A_B11",
    821. 

  821.               :cassette                              => "L1L2_gt2",
    822. 

  822.               :cassette_type                         => "Promotorless",
    823. 

  823.               :"mouse?"                              => "no",
    824. 

  824.               :qc_count                              => 3,
    825. 

  825.               :production_qc_five_prime_screen       => "pass",
    826. 

  826.               :production_qc_loxp_screen             => "not confirmed",
    827. 

  827.               :production_qc_three_prime_screen      => "pass",
    828. 

  828.               :production_qc_loss_of_allele          => "-",
    829. 

  829.               :production_qc_vector_integrity        => "-",
    830. 

  830.               :distribution_qc_karyotype_high        => "-",
    831. 

  831.               :distribution_qc_karyotype_low         => "-",
    832. 

  832.               :distribution_qc_copy_number           => "-",
    833. 

  833.               :distribution_qc_five_prime_lr_pcr     => "-",
    834. 

  834.               :distribution_qc_five_prime_sr_pcr     => "-",
    835. 

  835.               :distribution_qc_three_prime_sr_pcr    => "-",
    836. 

  836.               :distribution_qc_thawing               => "-",
    837. 

  837.               :user_qc_southern_blot                 => "-",
    838. 

  838.               :user_qc_map_test                      => "-",
    839. 

  839.               :user_qc_karyotype                     => "-",
    840. 

  840.               :user_qc_tv_backbone_assay             => "-",
    841. 

  841.               :user_qc_five_prime_lr_pcr             => "-",
    842. 

  842.               :user_qc_loss_of_wt_allele             => "-",
    843. 

  843.               :user_qc_neo_count_qpcr                => "-",
    844. 

  844.               :user_qc_lacz_sr_pcr                   => "-",
    845. 

  845.               :user_qc_five_prime_cassette_integrity => "-",
    846. 

  846.               :user_qc_neo_sr_pcr                    => "-",
    847. 

  847.               :user_qc_mutant_specific_sr_pcr        => "-",
    848. 

  848.               :user_qc_loxp_confirmation             => "-",
    849. 

  849.               :user_qc_three_prime_lr_pcr            => "-"
    850. 

  850.             },
    851. 

  851.             {
    852. 

  852.               :name
    853.